Diseño, montaje y prueba de un sistema compacto de microscopía holográfica digital en geometría de transmisión

Abstract

The main purpose of this work was to design and assemble a portable, lightweight, robust microscope with a greater field of vision in relation to a traditional microscope at Spectroscopy Laboratory, Department of Physics of the National Polytechnic School. As a solution, there is a microscopy system based on digital holography that works in two parts: 1) obtaining the interferogram produced by the interaction of the light source and the sample; 2) the numerical reconstruction of an image of the sample based on the interferogram.

The chosen holographic system to obtain the interferogram was digital holographic lensless microscopy in transmission geometry. A coherent light source illuminates the sample producing an interference pattern, formed due to the interaction of dispersed and non-dispersed waves. The system was mounted on a 3D printed structure and works with optically low dense samples, especially with particles, the system gives a field of vision equal to the active area of the sensor.