Evaluación de disruptores endocrinos, sobre bioindicadores acuáticos, para determinar índices de toxicidad : determinación de los índices de toxicidad cic50%, cig50%, cl50%, en bioindicadores acuáticos, a. Cepa, l. Sativa y d. Magna, expuestos al disruptor endocrino isoflavona de soya.

Abstract

The present curricular research study focused on determining the acute toxicity indices of both root growth inhibition (CIC50%), germination inhibition (CIG50%) and lethality concentration (LC50%), of the endocrine disruptor “soy isoflavone.” ”, on three test species (Allium cepa, Latuca sativa and Daphnia magna), using static acute toxicity tests in the laboratory with a duration between 48 and 120 hours, to evaluate the growth and survival of the test organisms. It began with the cultivation and acclimatization of the test organisms to laboratory conditions, and then exposed the organisms individually or in groups to aqueous solutions of different concentrations of soy isoflavone, including the positive and negative control groups. , for a specific exposure period. Once the exposure period was completed, the trial endpoints, which consisted of measuring mortality or inhibition, were determined in each test group. These results were analyzed graphically and statistically to calculate toxicity indices. Finally, the graphic and statistical results revealed that the CIC50% for the species Allium cepa was 74.46 mg/L, the CIG50% for the species Latuca sativa was 1.348 mg/L and the LC50% for the species Daphnia magna was 161.92 mg/L. In summary, it was demonstrated that the plant species Latuca sativa presented a greater sensitivity to the presence of soy isoflavone compared to the animal species Daphnia magna, which reflected a greater tolerance.